Welcome to the Brown University Flow Cytometry & Cell Sorter Facility. The purpose of this facility is to provide technical assistance to researchers at Brown and affiliated institutions by performing flow cytometry-based analysis and sorting. The facility has a 4-laser, 54-channel Cytek Aurora flow cytometer that is equipped with an automated sample loader for analysis of 96 well plates,
as well as a 3-laser, 38-channel Cytek Aurora CS cell sorter.

Related Core

The Rhode Island Hospital Stem Cells and Aging (SCA) COBRE Flow Cytometry Core’s function is to provide high quality,
cost effective, state-of-the-art flow cytometry and multiparameter cell sorting instrumentation and associated expertise
and services to all investigators in the SCA COBRE and to the general research community in Rhode Island. The Core has
a BD Sciences Influx Cell Sorter and LSRII Analyzer/Flow Cytometer. It also has a Fluidigm Helios (CyTOF) Mass
Cytometer. The Core operates the instruments, provides training for self-use and provides advise on experimental
design and data interpretation.

Learn more

Find us at CoresRI!

Data Analysis & Backup

Files and experiments will occasionally pile up on the SpectroFlow software which causes the program to run at slower speeds. For this reason, every month, old experiments are deleted off the program, and saved onto the workstations hard drive, where it will be backed up at the end of the day.

Individual users are responsible for their own data analysis and backing up of data (either by transfer to USB Flashdrive or CD/DVD). There is only one PC workstation with SpectroFlow software for data analysis and/or for setting up sort profiles, which is connected to the instrument. The facility staff is available by appointment to assist in data analysis using the Flowjo software.

Protocols

Listed are several protocols that may answer some typical questions.

The Cell Sorting Guidelines are basic instructions as to how to prepare your samples for sorting. The Flow Cytometry Guidelines are basic instructions for running samples for flow analysis that do not need to be sorted. Please keep in mind that it is still very helpful to filter your samples, especially samples that come from bone marrow or are clumpy cells lines.

Protocol Downloads

Cell Sorting Guidelines  Flow Cytometry Guidelines  

Please go to Brown University iLab
Login with your Brown credentials and select the Flow Cytometry Core from the drop-down menu. Then click on “schedule equipment” or “request services” and follow the instructions for reservations and billing.

New External and Commercial customers click here

For further information or to schedule an appointment time, contact Kevin Carlson at 401-863-6396 or send us an email.

Email Kevin Carlson     Email the Flow Cytometry Facility

FY25 Rates

Effective 11/18/24

*Rates for Brown and Rhode Island Academic and Hospital Affiliates

**Any cancellations with less than a 24 hour notice before scheduled start time will be charged 1 hour. No-shows will be charged for time reserved.

The Flow Cytometry and Sorting Facility is supported by NIH funding. The following acknowledgement must appear in any publication made possible by the work of the Flow Cytometry and Sorting Facility.

This project was supported in part by a grant from the National Center For Research Resources (NCRR) (1S 10RR021051) from the National Institutes of Health, Brown University's Division of Biology and Medicine and Provost's office.