Division of Biology and Medicine
BioMed Core Facilities

Transgenic and Genome Editing Facility

The Transgenic and Genome Editing Facility (TGEF) serves primarily the Brown University community, including its affiliated research institutes and hospitals, at a base fee-for-service. We are also open to other institutes with rates higher than the base cost.

Welcome to the Brown Transgenic & Genome Editing Facility!

We provide one-stop shop services for generating genetically modified (GM) mouse or rat models, covering project consultation, novel model design, reagents, model production, germline transmission, and genotyping. The deliverables of novel models are F1 heterozygous mice or rats, along with their genotyping analysis protocol.

The cutting-edge technologies of the CRISPR/Cas platform are primarily employed at the TGEF to accomplish the vast majority of projects in a cost-effective, fast, and high-quality manner. The conventional ES cell gene-targeting system is employed as a complementary approach to overcome the limitations of the CRISPR/Cas system. 

Routine services include genotype analysis, sperm or embryo cryopreservation and storage, mouse or rat (frozen embryos) line rederivation, in vitro fertilization (IVF). Other services, such as mouse vasectomy, embryo transfer, colony scale-up, and intracytoplasmic sperm injection (ICSI) are also available. New services requiring TGEF resources can be created after consulting with us. 

Address/Contact

Transgenic and Genome Editing Facility, Brown University

70 Ship Street, Room 205

Providence, Rhode Island, 02903

Phone: (401) 863-9544

Team

  • JL

    Jinping Luo

    Director, Transgenic Facility; BioMed/MCB Department

    Dr. Luo is responsible for production of user-defined transgenic mice and the overall management of the Mouse Transgenic and Gene Targeting Facility. He supervises the overall operation of the facility including cryopreservation and rederivation services, ensures facility compliance with IACUC regulations, and provides consultation to investigators.

  • ZP

    Zuping Qu

    Research Assistant, Transgenic Facility; BioMed/MCB department
  • Johannes Fimmers

    Johannes Fimmers

    Research Assisant, Transgenic Facility, BioMed/MCB department

Service Request and Reservations

Please go to Brown University iLab
Login with your Brown credentials and select the Transgenics and Genome Editing Core from the drop-down menu. Then click on “request services” and follow the instructions for reservations and billing.

New External and Commercial customers click here

For further information or if you require services that are not listed here, please contact Jinping Luo, the facility director.

Rates

FY25 Rates

ServiceUnitsInternal Academic Rates*External Academic
Not Rhode Island
CRISPR/Cas9
Guide RNA validationTarget site$613$977
Donor construction/preparation - Knock-In (KI, >80bp)Project$1,896$3,024
Donor construction/preparation - Conditional Knock-Out (cKO - two steps)Project$634$1,011
Donor construction/preparation - Point Mutation (PM)Project$166$265
Founder GenotypingSample$15$25
Germline TransmissionFounder$683$1,090
Embryo Microinjection/Electroporation 
Embryo Microinjection/Electroporation (B6)Project$4,435$7,073
Embryo Microinjection/Electroporation (FVB)Project$3,217$5,132
Embryo Microinjection/Electroporation (CD-1)Project$2,168$3,459
Cryopreservation 
Embryo Cryo (Homozygotes)Line$567$905
Embryo Cryo (Heterozygotes)Line$923$1,472
Sperm CryoLine$483$771
Mouse line Cryo Recovery
From EmbryoLine$1,185$1,889
From SpermLine$1,375$2,192
In Vitro Fertilization (IVF)
Fresh spermLine$1,546$2,465
Frozen spermLine$1,471$2,345
IVF plus Sperm CryoLine$2,018$3,319
IVF plus Embryo CryoLine$1,846$2,944
RederivationLine$1,646$2,625
Colony scale-upLine$2,018$3,319
Individual Services
Germline TransmissionMouse$688$1,097
Annual Cryo Storage FeeYear$111$178
Hourly Rate for custom projects not listedHour$75$120

*Rates for Brown and Rhode Island Academic and Hospital Affiliates. For External Academic or Commercial Rates, contact Jinping Luo.

Rates Effective 11/18/2024

Services and Instruments

Resources for Grants

Acknowledgment

This facility was supported in part by grants from the National Institutes of Health (NIH), Brown University's Division of Biology and Medicine and Provost's office.

The following acknowledgement must appear in any publication made possible by the work of the Transgenic Facility.

"This project was supported by the Mouse Transgenic and Gene Targeting Facility at the Brown University that was funded by a grant from the National Institute of General Medical Sciences (P30 GM103410) from the National Institutes of Health."

Links to Resources

CRISPR Design Tools

CRISPRscan is a novel scoring algorithm from the Giraldez Lab (Yale University) that helps you select the best gRNAs.
CHOPCHOP (version 3) is a web tool for selecting target sites for CRISPR/Cas9, CRISPR/Cpf1, CRISPR/Cas13 or NICKASE/TALEN-directed mutagenesis.
CRISPOR is a program that helps design, evaluate and clone guide sequences for the CRISPR/Cas9 system.
A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases.
The CRISPR Guide RNA design tool allows you to visualize, optimize, and annotate multiple gRNA sequences at a time.
World’s Fastest & Easiest CRISPR Gene Knockout Design Tool
To avoid unwanted in-frame deletions in a protein-coding sequence as much as possible,
one should choose target sites with high out-of-frame scores.

ES Cells

The following are sources for constructs, Genetrap ES cells, and WT ES cells. If you are considering using cells from these sources for microinjection at Brown, please contact the facility prior to purchase.

Mouse Model Nomenclature

Montoliu Lluís & Whitelaw C. Bruce A. (2010) Using standard nomenclature to adequately name transgenes, knockout gene alleles and any mutation associated to a genetically modified mouse strainTransgenic Research (ePub, 16 July 2010)

"The International Society for Transgenic Technologies (ISTT) and the scientific journal, Transgenic Research, to which the ISTT is associated, have decided to promote the use of standard nomenclature for naming mouse strains, genes, mutations and alleles. In this regard, and according to ISTT aims and activities, ISTT will disseminate in its web siteblog, newsletter and Transgenic Technology (TT) meetingsinformation and recommendations to use standard nomenclature. Likewise, the correct use of rules and guidelines for standard nomenclature for naming mouse strains, genes, mutations and alleles will be enforced in subsequent articles submitted to Transgenic Research for publication, as it is already done in several other journals."Nomenclature_Transgenic or KO mouse line

 

https://www.jax.org/jax-mice-and-services/customer-support/technical-support/genetics-and-nomenclature

 

mouse nomenclature_Jax