Division of Biology and Medicine
BioMed Core Facilities

X-Ray Source

The facility's X-ray instrumentation is built around a Rigaku MicroMax 003 X-ray generator.

Crystal Diffraction

Our system is fitted with a Saturn 944 HG CCD for detecting crystal diffraction. The goniometer is equipped with a Micro-glide 3-axis head that allows automated centering. Crystals can be mounted manually, but the facility also has an ACTOR robot that can mount crystals from up to 5 ALS or Uni pucks. With this system, up to 80 crystals can be automatically screened in a single run. The robot can then collect the best samples on a single puck for a synchrotron trip, or devise and execute a strategy for in-house data collection.

Methods Library

Crystallization and Condition Screening

Calero G, Cohen AE, Luft JR, Newman J, and Snell EH. "Identifying, studying and making good use of macromolecular crystals". Acta Cryst. F 2014 (F70): p 993-1008. DOI: 10.1107/S2053230X14016574

Collecting Diffraction Data

Mueller M, Wang M, and Schulze-Briese C. "Optimal fine φ-slicing for single-photon-counting pixel detectors". Acta Cryst. D 2012 (D68): p 42-56. DOI: 10.1107/S0907444911049833

Krojer T, Pike ACW, and von Delft F. "Squeezing the most from every crystal: the fine details of data collection." Acta Cryst. D 2013 (D69): p 1303-13. DOI: 10.1107/S0907444913013280