The 850 MHz ASCEND spectrometer was installed in 2013 and is equipped with a cryoprobe and Avance III HD console. The spectrometer has four full channels and a TCI HCN z-gradient cryoprobe. The instrument is controlled using a Linux workstation running TopSpin 3.5. A room-temperature probe is also available; both probes have automatic tuning and matching capability.
Division of Biology and Medicine
BioMed Core Facilities
850 MHz Bruker Avance III HD
850 MHz Bruker Avance III HD
Methods Library
Chemical Exchange
Wang C, Rance M, Palmer AG III. "Mapping chemical exchange in proteins with MW > 50 kD." J Am. Chem. Soc. 2003 (125): p 8968-9. DOI: 10.1021/ja035139z
Kneller JM, Lu M, and Bracken C. "An effective method for the discrimination of motional anisotropy and chemical exchange". J. Am. Chem. Soc. 2002 (124): p 1852-3. DOI: 10.1021/ja017461k
Dynamics Models
Lipari G and Szabo A. "Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 1. Theory and range of validity". J. Am. Chem. Soc. 1982 (104): p 4546-4559. DOI: 10.1021/ja00381a009
1
of
3
2
of
3
3
of
3