Division of Biology and Medicine
BioMed Core Facilities

850 MHz Bruker Avance III HD

The 850 MHz ASCEND spectrometer was installed in 2013 and is equipped with a cryoprobe and Avance III HD console.  The spectrometer has four full channels and a TCI HCN z-gradient cryoprobe. The instrument is controlled using a Linux workstation running TopSpin 3.5. A room-temperature probe is also available; both probes have automatic tuning and matching capability.

Methods Library

Chemical Exchange

Wang C, Rance M, Palmer AG III. "Mapping chemical exchange in proteins with MW > 50 kD." J Am. Chem. Soc. 2003 (125): p 8968-9. DOI: 10.1021/ja035139z

Kneller JM, Lu M, and Bracken C. "An effective method for the discrimination of motional anisotropy and chemical exchange". J. Am. Chem. Soc. 2002 (124): p 1852-3. DOI: 10.1021/ja017461k

Dynamics Models

Lipari G and Szabo A. "Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 1. Theory and range of validity".  J. Am. Chem. Soc. 1982 (104): p 4546-4559. DOI: 10.1021/ja00381a009