A Bruker NEO 600 MHz spectrometer was installed in 2018 for solution-state protein NMR experiments. It is equipped with a triple-resonance inverse detection cryoprobe with cooled 1H, 13C and 15N preamplifiers. A room temperature probe is also available for this instrument. It is operated using software Topspin 4.0.
Division of Biology and Medicine
BioMed Core Facilities
600 MHz Bruker NEO
600 MHz Bruker NEO
Methods Library
Chemical Exchange
Wang C, Rance M, Palmer AG III. "Mapping chemical exchange in proteins with MW > 50 kD." J Am. Chem. Soc. 2003 (125): p 8968-9. DOI: 10.1021/ja035139z
Kneller JM, Lu M, and Bracken C. "An effective method for the discrimination of motional anisotropy and chemical exchange". J. Am. Chem. Soc. 2002 (124): p 1852-3. DOI: 10.1021/ja017461k
Dynamics Models
Lipari G and Szabo A. "Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 1. Theory and range of validity". J. Am. Chem. Soc. 1982 (104): p 4546-4559. DOI: 10.1021/ja00381a009