Division of Biology and Medicine
BioMed Core Facilities

600 MHz Bruker NEO

A Bruker NEO 600 MHz spectrometer was installed in 2018 for solution-state protein NMR experiments. It is equipped with a triple-resonance inverse detection cryoprobe with cooled 1H, 13C and 15N preamplifiers. A room temperature probe is also available for this instrument. It is operated using software Topspin 4.0.

Methods Library

Chemical Exchange

Wang C, Rance M, Palmer AG III. "Mapping chemical exchange in proteins with MW > 50 kD." J Am. Chem. Soc. 2003 (125): p 8968-9. DOI: 10.1021/ja035139z

Kneller JM, Lu M, and Bracken C. "An effective method for the discrimination of motional anisotropy and chemical exchange". J. Am. Chem. Soc. 2002 (124): p 1852-3. DOI: 10.1021/ja017461k

Dynamics Models

Lipari G and Szabo A. "Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 1. Theory and range of validity".  J. Am. Chem. Soc. 1982 (104): p 4546-4559. DOI: 10.1021/ja00381a009