Division of Biology and Medicine
BioMed Core Facilities

Microcal Isothermal Titration Calorimetry (ITC) 200


Microcal iTC200Isothermal Titration Calorimetry (ITC) is the gold standard for measuring biomolecular interactions.  ITC simultaneously determines all binding parameters (n, K, ∆H and ΔS) in a single experiment – information that cannot be obtained from any other method.

When substances bind, heat is either generated or absorbed.  ITC is a thermodynamic technique that directly measures the heat released or absorbed during a biomolecular binding event.  Measurement of this heat allows accurate determination of binding constants (K), reaction stoichiometry (n), enthalpy (∆H) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment.  Because ITC goes beyond binding affinities and can elucidate the mechanism of the molecular interaction, it has become the method of choice for characterizing biomolecular interactions.



Applications include:

  • Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.
  • Lead optimization.
  • Enzyme kinetics.
  • Assessment of the effect of molecular structure changes on binding mechanisms.
  • Assessment of biological activity.
  • Interactions between any two molecules can be studied with ITC, including:

  • Protein-small molecule
  • Protein-protein
  • Target-drug
  • Enzyme-inhibitor
  • Antibody-antigen
  • Protein-DNA
  • Protein-lipid
  • Small molecule-small molecule


Please e-mail Mandar_Naik@Brown.edu to gain the ability to book an instrument through our calendar system.

To view the availability of this instrument, please click on the link below:

Microcal ITC200