Division of Biology and Medicine
BioMed Core Facilities

Molecular Pathology Core

The Molecular Pathology Core provides all-inclusive histology and histopathology services, equipment and training. The core houses an automated tissue processor, a paraffin embedding center, automated microtomes, a cryostat, a vibratome and a multiheaded light microscope.

About

The Molecular Pathology Core at Brown University, open to all investigators, provides all-inclusive histology and histopathology services and training. The core houses state-of-the-art equipment, including an automated tissue processor, a paraffin embedding center, two automated microtomes, a cryostat, a vibratome for soft-tissue sectioning, a multiheaded light microscope with projection capabilities and a slide scanner with analysis software for the identification and quantification of morphological structures.  The Molecular Pathology Core is staffed by a director, a histologist and a histotechnician, who have expertise in histopathological and immunocytochemial methods, including fixation, dehydration, embedding, sectioning, histological staining, immunolabeling, high-resolution imaging, and quantitative image analysis. Facility staff provides all-inclusive services in sample preparation, offers assistance in imaging and image analysis and provides consultation for ongoing or future research projects. 

The core is listed in CoresRI.org, a searchable directory of core facilities in Rhode Island. 

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Services and Instruments

Tissue Sample Processing

  • Paraffin Processing and Embedding
  • Plastic Processing and Embedding
  • Frozen Tissue-Embedding in OCT Block
  • TEM Processing and Embedding
  • SEM Processing
  • Sample Preparation for Serial Blockface Imaging

Staining

  • Hematoxylin and Eosin (H&E)
  • Special Stains*

*Special Stains include Masson's Trichrome, Sirius Red, Congo Red, Oil Red O., Silver stains and other heavy metals, Periodic Acid Schiff (PAS)

Sectioning

  • Paraffin
  • Plastic
  • Frozen/Cryostat
  • Ultrathin
  • Vibratome
  • Serial and Step Sectioning

Immunolabelling

  • Immunohistochemistry, Immunocytochemistry
  • Immunofluorescence

Additional Services

  • Technical Assistance
  • Consultation and Support Services

Instrumentation

Instrument/Service Manufacturer Function
Automated Tissue Processor Leica ASP300 S  Tissue processing for paraffin embedding
Paraffin Embedding Center Leica EG1150C  Paraffin embedding
Automated Microtome Microm HM 355S  Paraffin sectioning
Automated Microtome Leica RM2265 Paraffin sectioning, plastic sectioning
Cryostat Leica CM3050S  Sectioning of fixed or unfixed frozen samples
Vibratome Leica VT 1000S  Sectioning of fixed or unfixed samples
Light microscope with a projector Nikon Eclipse 50i  Collaborative analyses of histological slides and digital imaging capability
-80C Freezer Revco Sample storage

Protocols

Osmium-Thiocarbohydrazide-Osmium (OTO) Enhanced Contrast Procedure

Osmium-Thiocarbohydrazide-Osmium (OTO) Epon TEM Processing Protocol

Other Protocols

Gatan-Sample Prep, SEM for Serial Block-Face Imaging

Service Request and Reservations

To request a service, please contact David Silverberg,
Telephone: (401) 863-6151

Scheduling

ATTENTION! Please see the link to the Molecular Pathology Core Calendar for scheduling.

Reservation Instructions: Molecular Pathology Core

  1. Open your Google Calendar through your Brown Gmail account.
  2. Create an event by either:
    1. a. clicking the “Create” button on the top left of your calendar
      or
    2. clicking on the area in the calendar (Date and Time) that you want to reserve.
  3. Add the event name and ensure the correct date and time.
  4. Copy and paste the following Google Calendar ID into the “Add Guests” field.
    Calendar ID: brown.edu_bljg99rnt3gllarumk62osgus8@group.calendar.google.com
  5. Click the "Save" button once you are finished.

Rates

FY22 Rates

Service

Units

Internal Academic Rates*

External Academic

Tissue Sample Processing

Paraffin Processing and Embedding - automated Cassette $10 $15
Paraffin Processing and Embedding - manual Cassette $20 $33
Placing tissue in cassettes Cassette $5 $8
EM processing - first sample** Cassette $100 $159
EM processing - extra samples** Cassette $14 $22
High Contrast EM  - first sample Cassette $240 $381
High Contrast EM  - extra samples Cassette $15 $24

Sectioning

Paraffin sectioning - first slide Slide $12 $19
Paraffin sectioning - extra slides Slide $4 $6
Plastic sectioning - first slide Slide $15 $23
Plastic sectioning - extra slides Slide $7 $11
Frozen Tissue - Embedding in OCT Block Cassette $15 $24
Frozen sectioning - first slide Slide $23 $37
Frozen sectioning - extra slides Slide $6 $10
TEM ultrathin sectioning Hour $80 $127

Staining

H&E staining - first slide Slide $9 $14
H&E staining - extra slides Slide $6 $9
Special Staining - first slide Slide $18 $29
Special Staining - extra slides Slide $11 $17

Immunolabeling

Immunohistochemistry - first slide Slide $65 $103
Immunohistochemistry - extra slides Slide $8 $14

Technical Assistance

Labor and Technical Assistance Hour $36 $58

Effective 10/1/2021

*Rates for Brown and Rhode Island Academic and Hospital Affiliates

**Enhanced contrast EM: enhanced staining for serial block-face imaging on the Thermo Apreo VS SEM

Please see FAQ section for requirements for tissue placed in cassettes prior to submission to the molecular pathology core.

Contacts/Locations

Faculty Advisor

Resources for Equipment

Instruments and Facilities

The Leduc Bioimaging Facility provides equipment and training dedicated to high-resolution imaging in the life sciences.
Visit Page

Resources for Grants

Molecular Pathology Facility. This facility, located at the Laboratories for Molecular Medicine, is supervised by an MD-PhD-level, board certified pathologist and staffed by a full-time manager and research assistant. The facility provides access to histopathological, immunohistochemical and immunocytological technologies. Processing, embedding, sectioning, and staining of specimens is provided. The instrumentation includes a Leica EG1160 embedding center, a Leica CM3050S cryostat, a Micron HM355 rotary microtome for paraffin sections, a Leica RM2265 rotary microtome for thick and semi-thin plastic sections and paraffin sections, a Leica ASP300 S Automated Vacuum Tissue Processor, a ScanScope CS digital slide scanning system from Aperio Technologies, a Fujix Bas 1000 phosphor imager, a Nikon Eclipse TS100 inverted fluorescence microscope, a Nikon E800 microscope with a digital camera, a Leica VT1000S Vibratome for soft tissue sectioning, and an Arcturus PixCell II laser capture microdissection system.

Scientific reproducibility is enhanced through scientific rigor and transparency.  Scientific rigor is the strict application of the scientific method to ensure unbiased and well-controlled experimental design, methodology, analysis, interpretation, and reporting of results. The Molecular Pathology Core is committed to supporting research excellence by adopting the following practices of scientific rigor.

  • Purchase and maintain a variety of high-quality instruments from established vendors such that the best instrument is available for any given research analysis.
  • The equipment is overseen by highly trained molecular pathology and histology experts and well maintained under service contracts or funds budgeted for annual preventive maintenance and repairs.
  • The molecular pathology and histology experts are available for experimental design consults or troubleshooting.

FAQ

How large should tissue sample sizes be?

For routine processing, sections should be no more than 3mm thick. A section should not touch both the top and bottom of the tissue-processing cassette.

Acknowledgment

This facility was supported in part by grants from the National Institutes of Health (NIEHS Grant Number P42ES013660), Brown University's Division of Biology and Medicine and Provost's office.

Superfund Research Program:  The Molecular Pathology Core began as part of Brown University's Superfund Research Program (SRP) - Toxicant Exposures in Rhode Island: Past, Present, and Future. The Core's initial purpose was to examine molecular and morphological changes in cells, tissues, and organs following exposure to complex environmental contaminants. The core was opened to non-superfund users soon after to stimulate interdisciplinary collaborations in environmental toxicology and create critical mass in the development of novel methodologies.