Division of Biology and Medicine
BioMed Core Facilities
Next Generation Sequencing
A high throughput bench top sequencer that provides accurate fast sequencing data, and can be easily adopted to a researcher’s needs depending on their required read length and sequencing depth.
Next Generation Sequencing
A high throughput bench top sequencer that provides accurate fast sequencing data, and can be easily adopted to a researcher’s needs depending on their required read length and sequencing depth.
Overview
The Genomics Cores uses an Illumina NextSeq 550 sequencer in combination with Illumina v 2.5 sequencing reagents.
This system is a high throughput bench top sequencer that provides accurate fast sequencing data, and by employing flexible run configurations it can be easily adopted to a researcher’s needs depending on their required read length and sequencing depth.
Reagents are available in a mid-output and a high output mode resulting in maximum cluster numbers of 400 million (high output) and 130 million (mid output) clusters (reads).
The following reagent kits are available:
| Mid Output | High Output |
|---|---|
| N/A | 75 Cycles |
| 150 Cycles | 150 Cycles |
| 300 Cycles | 300 Cycles |
Each flow cell is a ‘single lane’ experiment which means that as soon as a researcher’s samples passed quality control the samples can be run on the sequencer as soon as it is available. Each reagents kit contains extra reagents sufficient for possible index reads.
Before a run, samples will undergo quality control, using the fragment analyzer for analysis of the libraries size distribution and to exclude adaptor contamination. qPCR analysis will be used to accurately determine the absolute concentration of the samples. Using this information sample concentration will be adjusted to the optimal concentration for clustering.
During each run a low amount of PhiX library will be included for quality control of the sequencing run.
Sequencing data can be delivered to a secure cloud location (Basespace) or to Brown’s super computer (OSCAR).
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Resources
Illumina Websites
Reservations
This is a full service instrument. Please coordinate with the facility manager about sample drop off.
The cost for sequencing reagents and quality control is not included in this price.
For more information or to request service, please contact the director of the Genomics Core, Christoph Schorl (Christoph_Schorl@brown.edu, 401-863-2875).
SEQUENCING GUIDELINES SAMPLE SUBMISSION FORM
The University of Rhode Island Genomics and Sequencing Center (RIGSC) is available to provide Sanger Sequencing and MiSeq Next Generation Sequencing services to Brown University researchers. Contact the RIGSC manager for additional information.