Division of Biology and Medicine
BioMed Core Facilities
Li-Cor Odyssey Clx Infrared Fluorescent Western Scanning / Imaging System
Li-Cor Odyssey Clx Infrared Fluorescent Western Scanning / Imaging System
Overview
The Odyssey Infrared Imaging System allows multiplex Western Blotting and accurate protein detection and quantification using secondary antibodies labeled with IRDye near-infrared (NIR) fluorescent dyes.
Applications
- Quantitative Western Blots
- In-Cell Western™ Assay
- Viral Titration Assays
- On-Cell Western Assay
- Tissue Section Imaging
- RNAi Analysis with NIR Fluorescence
- Protein Gels
- EMSA/Gel Shift Assay
- Glycoprotein Detection
- In-Gel Western
- Nucleic Acid Gel Imaging
- Protease Assay
- Protein Arrays
Operation
Specifications
| Laser/Microscope | |
|---|---|
| Image Field Size | 25 cm × 25 cm |
| Dynamic Range* | Manual: 16 bit (>4 logs), Auto: 22 bit (>6 logs) |
| Laser Lifetime | 40,000 hours typical |
| 700 Channel Laser Source | Solid-state diode laser at 685 nm |
| 800 Channel Laser Source | Solid-state diode laser at 785 nm |
| Detectors | Silicon avalanche photodiodes |
| Scanning Speed | 5-40 cm/s |
| Resolution | 21-337 µm |
| Focusing Range | Microscope is adjustable 0 mm-4 mm above the scan bed to obtain best signal-to-noise ratio. |
| * This dynamic range is obtained in a single acquisition. |
| Operation Specifications | |
|---|---|
| Operating Conditions | 15-35 °C and dew point no greater than 20 °C |
| Power Requirements | Universal input range is between 100-240 VAC (voltage fluctuations not to exceed 10% of the nominal voltage); 4 Amp maximum; at 120V, 0.5 Amp typical when idle; 50/60 Hz. |
| Size and Weight | |
|---|---|
| Dimensions (instrument only) | 53 cm W × 62 cm D × 37 cm H |
| (21" × 24.5" × 15") | |
| Height with hood fully open is 74 cm (29") | |
| Weight | 33 kg (72 lbs) |
| Software Specifications* | |
|---|---|
| Empiria Studio® Software | Requires a minimum of 8 GB of RAM; 64-bit versions of Windows 7 or 10; Mac OS Sierra or High Sierra |
| Image Studio™ Software | For operating system compatibility, see this table. |
*Please contact LI-COR for compatibility with other software packages.
Resources
Reservations
Instrument Reservation
Internal users can reserve an instrument. For all other instruments and services, please contact the Facility Manager.