Division of Biology and Medicine
BioMed Core Facilities

Zeiss Lumar V12 Fluorescence Stereomicroscope

Overview

Sidney Frank Hall, Room 113

The Zeiss Lumar V12 Fluorescence Stereomicroscope

The Zeiss Lumar V12 Fluorescence Stereomicroscope is capable of imaging transmitted light, reflected light, and fluorescence of larger samples (field of view is 1.4 x 1.1 cm). The microscope has a motorized focus and zoom, with foot pedals for hands-free operation. The motorized filter turret allows one to collect brightfield and multiple colors of fluorescence in timelapse mode. The microscope is equipped with filters for imaging DAPI, FITC / GFP, and RHOD and has a high NA objective (ApoLumar S 1.2x) and sensitive monochrome camera (AxioCam MRm), which makes this system suitable for imaging low levels of fluorescence.

Operation

Lumar Filters

DAPI (485049):  excitation 365 nm, emission 445/50 nm* 

GFP (486038): excitation 470/40 nm, emission 525/50 nm

Rhod (486043): excitation 550/25 nm, emission 605/70 nm

* 000/00: the first number is the midpoint of the band pass filter, the second number is the bandwidth of the filter.  E.g. Rhod 605/70 collects emitted light from 570 - 640 nm.

Resources

Reservations

Microscopes in the Bioimaging Facility can be reserved using iLab calendars. All facility users are invited to use the system, which requires a one-time registration.

Contact