Zeiss LSM 880 Confocal Laser Scanning Microscope
Optical sectioning of samples labeled with fluorescent indicators
Zeiss LSM 880 Confocal Laser Scanning Microscope
Optical sectioning of samples labeled with fluorescent indicators
Overview
Laboratories for Molecular Medicine, Room 133
The Zeiss LSM 880 confocal laser scanning microscope is designed for imaging fluorescence, either in fixed or live samples. A pinhole removes the out-of-focus light and allows investigators to acquire thin optical sections at various focal planes. Stacks of images can be acquired for 3D visualization.
Operation
Scanhead. The system has a Quasar module scanhead - 32 channel GaAsP detector and two flanking multialkali PMTs. The 32-channel spectral detector allows unmixing of multiple dyes on-the-fly with a scan of a single frame. Two additional channels can be acquired with the flanking detectors optimized for imaging in deep blue or far red spectra. Both the spectral GaAsP and far-red MA PMT are liquid-cooled keeping the noise very low and thus increasing sensitivity of the system.
Lasers. The system has a unique set of lasers. Besides the typical Argon laser (458/488/514 nm for CFP/GFP/YFP), diode 561 nm laser and a He-Ne 633 laser, the system is also equipped with the HeNe 594 nm laser (mCherry, mPlum), and pulsed high-power 405 nm and 440 nm lasers that can be used for ablation and photoactivation.
Objectives. This LSM 880 has a set of premium objectives for a broad range of applications:
LD C-Apochromat 40x/1.1 W Corr UV-VIS-IR. This objective has a unique working distance of ~600 um for imaging large volumes, e.g. cleared specimen.
Plan-APOCHROMAT 100x/1.4 Oil Ph3. An objective for fluorescence and phase contrast imaging
Plan-Apochromat 40x/1.3 and 63x/1.4 Oil DIC UVVIS-IR. Both lenses are routinely used for high-resolution imaging and are corrected over a broad range of spectral lines from UV to IR.
Finally, two highly corrected air (no immersion) objectives Plan-Apochromat 20x/0.8 WD=0.55 M27 and Plan-Apochromat 10x/0.45 WD=2.0 M27.