Division of Biology and Medicine
BioMed Core Facilities

Zeiss LSM 710 Confocal Laser Scanning Microscope

Optical sectioning of samples labeled with fluorescent indicators

Breadcrumb

Bioimaging Facility

Zeiss LSM 710 Confocal Laser Scanning Microscope

Optical sectioning of samples labeled with fluorescent indicators

Overview

Sidney Frank Hall, room 113

The Zeiss LSM 710 confocal laser scanning microscope is designed for imaging fluorescence, either in fixed or live samples. A pinhole removes the out-of-focus light and allows investigators to acquire thin optical sections at various focal planes. Stacks of images can be acquired for 3D visualization.

The confocal module is mounted on a Zeiss Axio Observer Z1 inverted microscope with high quality objectives (listed below). A heated stage is available for imaging live samples at 37°C. The microscope has multiple lasers for excitation at 405, 458, 488, 514, 561, 594, and 633 nm, and is equipped with a 34-channel QUASAR detector, which can be set to collect emitted light of any wavelength within the visible spectrum. The QUASAR detector is capable of acquiring spectral information and its flexibility allows imaging of a broad range of fluorescent indicators, including the Alexa-Fluors, Calcium-Green, Cy2, Cy3, Cy5, DAPI, Fluorescein (FITC), FM1-43, Fluo-3, Fluo-4, GFP, GFP variants (BFP, CFP, EGFP, YFP), Hoechst 33258, Rhodamine, Texas Red, TRITC, and others. Images are acquired with ZEN 2008 software and can be stored on a CD, DVD, or a flash drive via a USB connection. The LSM images can be opened using a freely downloadable ZEN viewer.

Operation

Objectives on the 710:

Mag

Correction

NA

DIC

WD (mm)

Coverslip #

10x

Plan-Apochromat

0.45

-

2.0

0.17mm #1 1/2

20x

Plan-Apochromat

0.8

DIC

0.55

0.17mm #1 1/2

40x W

C-Apochromat

1.2

DIC

0.28

0.17mm #1 1/2

63x Oil

Plan-Apochromat

1.40

DIC

0.11

0.17mm #1 1/2

Theoretical XY Objective Resolution*: based on: Dxy=0.61*wavelength/NA

Mag

NA

~460 nm (Dapi)

~535 nm (Fitc)

~620 nm (Rho)

~720 nm (Cy5)

10x

0.45

625 nm

725 nm

840 nm

975 nm

20x

0.8

350 nm

410 nm

475 nm

550 nm

40x W

1.2

235 nm

270 nm

315 nm

365 nm

63x Oil

1.40

200 nm

230 nm

270 nm

315 nm

Theoretical Z Objective Resolution*: based on: Dz=wavelength/(NA*NA)

Mag

NA

~460 nm (Dapi)

~535 nm (Fitc)

~620 nm (Rho)

~720 nm (Cy5)

10x

0.45

2270 nm

2640 nm

3060 nm

3555 nm

20x

0.8

720 nm

835 nm

970 nm

1125 nm

40x W

1.2

320 nm

370 nm

430 nm

500 nm

63x Oil

1.40

235 nm

270 nm

315 nm

370 nm

Reservations

Reserve a microscope using the iLab calendars

Reserve a microscope using the iLab calendars

Reserve a microscope

Publications

The Zeiss 710 Laser Scanning Confocal Microscope was purchased with a high-end instrumentation grant from the Office of the Director at the National Institutes of Health (S10RR023693).

Contact

Bioimaging Facility