Division of Biology and Medicine
BioMed Core Facilities

Philips 410 Transmission Electron Microscope

Transmission electron microscopy with Advanced Microscopy Techniques (AMT) Camera Accessory

Overview

Sidney Frank Hall, Room 105

The Philips 410 Transmission Electron Microscope

The Philips 410 transmission electron microscope is equipped with a1k x 1k Advantage HR CCD camera from Advanced Microscopy Techniques (AMT). Images are acquired and analyzed with AMT's imaging software.

Operation

Resources

The Molecular Pathology Core provides all-inclusive histology and histopathology services, equipment and training. The core houses an automated tissue processor, a paraffin embedding center, automated microtomes, a cryostat, a vibratome and a multiheaded light microscope.

Reservations

Microscopes in the Bioimaging Facility can be reserved using iLab calendars. All facility users are invited to use the system, which requires a one-time registration.

1. Go to our facility's iLab site: ILABS
https://brown.ilab.agilent.com/service_center/show_external/5278/bioimaging_facility
2. In the upper-right-hand corner of the screen click ‘Sign In,’ and select to sign in using Brown University credentials 
3. You will be directed to an authentication page where you will enter your Brown University credentials
4. Once you have entered your credentials, click the ‘Login’ button
5. You will be directed to an iLab Registration page where you will select your PI/Lab and verify your contact information. 
6. Once your registration has been submitted, your PI will receive a notification that you have requested membership to their lab in iLab.  They will need to approve your membership.
7. Your training status needs to be updated.  This is done on a rolling basis by Eric Olson (Eric_Olson@brown.edu) and Sandra Van Wagoner (sandra_van_wagoner@brown.edu).   Alternatively, you can email Geoffrey_Williams@brown.edu or Robbert_Creton@brown.edu asking for an update of the training status in iLab, listing the microscopes that you have been trained on in the Bioimaging Facility. 

When registration is completed (including step 6-7), you can schedule equipment using the iLab calendars.
1. Navigate to the core page: ILABS BIOIMAGING
    https://brown.ilab.agilent.com/service_center/show_external/5278/bioimaging_facility 
2. In the upper-right-hand corner of the screen click ‘Sign In,’ and select to sign in using Brown University credentials
3. You will be directed to an authentication page where you will enter your Brown University credentials
4. Once you have entered your credentials, click the ‘Login’ button
5. Select the Schedule Equipment tab and click on the ‘View Schedule’ button next to the instrument of interest. Click and drag on the time frame you would like to schedule your reservation for.
6. A window will pop up that will allow you to verify your reservations details and provide payment information before saving the reservation.

When it is time to use the microscope

1.  Open iLab and select 'Go to Kiosk'
2.  Start end End microscope session (Start is needed to run the microscope) 

Additional help

More detailed instructions can be found on the Help Site by clicking on the “HELP” link in the upper right hand corner. For any questions not addressed in the Help site, click on the “HELP” link in the upper right hand corner and submit a ticket, or email ilab-support@agilent.com.

Contact