Division of Biology and Medicine
BioMed Core Facilities

Olympus FV3000 Confocal Microscope

Optical sectioning of samples labeled with fluorescent indicators. Equipped with a resonant scanner for fast optical sectioning

Overview

Sidney Frank Hall, room 111

FV3000This confocal microscope can acquire thin optical slices of fluorescent samples. It has an inverted microscope configuration and is equipped with a resonant scanner for fast optical sectioning and 4 detectors, two standard Metal Alkalide and two high sensitivity GaAsP detetors.  

  • LASER DIODE 405NM,CW 50MW
  • LASER DIODE 488NM,CW 20MW
  • LASER DIODE 561NM,CW 20MW
  • LASER DIODE 640NM,CW 40MW

 

 

Operation

Objectives on the FV3000RS:

Mag

Correction

NA

HFW @ 1x

WD (mm)

Immersion Medium

1.25x

UPlan Apochromat

0.04

10.18 mm

5.0

Air/Dry

10x

UPlan Super Apochromat

0.4

1.27 mm

3.1

Air/Dry

20x

UPlan Super Apochromat

0.75

0.600 mm

0.60

Air/Dry

30xS

UPlan Super Apochromat

1.05

0.424 mm

0.80

Silicone Oil

60xS2

UPlan Super Apochromat

1.3

0.212 mm

0.30

Silicone Oil

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