Division of Biology and Medicine
BioMed Core Facilities
Nikon Ti2-E Fluorescence Microscope for High-content Imaging
High-content imaging system, based on an inverted microscope and equipped for imaging standard slides, culture dishes, and microplates (e.g. 96-well plates).
Nikon Ti2-E Fluorescence Microscope for High-content Imaging
High-content imaging system, based on an inverted microscope and equipped for imaging standard slides, culture dishes, and microplates (e.g. 96-well plates).
Overview
Laboratories for Molecular Medicine, Room 111
The Nikon Ti2-E fluorescence microscope is a high-content imaging system, based on an inverted microscope and equipped for imaging standard slides, culture dishes, and microplates (e.g. 96-well plates). The microscope includes 'Perfect Focus' for autofocus applications, a Photometrics Prime 95B sCMOS camera for imaging fluorescence, a 16 megapixel Nikon DS-Ri2 color camera for imaging histology slides, a motorized stage and NIS-Elements High Content (HC) software for multiwell plate acquisition.
Operation
Objectives:
- 4x Plan Apochromat Lambda, N.A. 0.2, W.D. 20 mm, F.O.V. 25 mm
- 10x Plan Fluor Phase Contrast DL, N.A. 0.3, W.D. 16 mm, F.O.V. 25 mm
- 20x Super Plan Fluor ELWD, N.A. 0.45, W.D. 6.9-8.2 mm, F.O.V. 22 mm, DIC, Correction Collar 0-2 mm
- 40x Super Plan Fluor ELWD, N.A. 0.6, W.D. 2.8-3.6 mm, F.O.V. 22 mm, DIC, Correction Collar 0-2 mm
- 60x Oil Plan Apochromat Lambda Phase Contrast DM, N.A. 1.4, W.D. 0.13 mm, F.O.V. 25 mm, Ph3
Resources
Nikon
Reservations
Microscopes in the Bioimaging Facility can be reserved using iLab calendars. All facility users are invited to use the system, which requires a one-time registration.
1. Go to our facility's iLab site: ILABS
https://brown.ilab.agilent.com/service_center/show_external/5278/bioimaging_facility
2. In the upper-right-hand corner of the screen click ‘Sign In,’ and select to sign in using Brown University credentials
3. You will be directed to an authentication page where you will enter your Brown University credentials
4. Once you have entered your credentials, click the ‘Login’ button
5. You will be directed to an iLab Registration page where you will select your PI/Lab and verify your contact information.
6. Once your registration has been submitted, your PI will receive a notification that you have requested membership to their lab in iLab. They will need to approve your membership.
7. Your training status needs to be updated. This is done on a rolling basis by Eric Olson (Eric_Olson@brown.edu) and Sandra Van Wagoner (sandra_van_wagoner@brown.edu). Alternatively, you can email Geoffrey_Williams@brown.edu or Robbert_Creton@brown.edu asking for an update of the training status in iLab, listing the microscopes that you have been trained on in the Bioimaging Facility.
When registration is completed (including step 6-7), you can schedule equipment using the iLab calendars.
1. Navigate to the core page: ILABS BIOIMAGING
https://brown.ilab.agilent.com/service_center/show_external/5278/bioimaging_facility
2. In the upper-right-hand corner of the screen click ‘Sign In,’ and select to sign in using Brown University credentials
3. You will be directed to an authentication page where you will enter your Brown University credentials
4. Once you have entered your credentials, click the ‘Login’ button
5. Select the Schedule Equipment tab and click on the ‘View Schedule’ button next to the instrument of interest. Click and drag on the time frame you would like to schedule your reservation for.
6. A window will pop up that will allow you to verify your reservations details and provide payment information before saving the reservation.
When it is time to use the microscope
1. Open iLab and select 'Go to Kiosk'
2. Start end End microscope session (Start is needed to run the microscope)
Additional help
More detailed instructions can be found on the Help Site by clicking on the “HELP” link in the upper right hand corner. For any questions not addressed in the Help site, click on the “HELP” link in the upper right hand corner and submit a ticket, or email ilab-support@agilent.com.