Division of Biology and Medicine
BioMed Core Facilities

Nikon Ti2-E Fluorescence Microscope for High-content Imaging

High-content imaging system, based on an inverted microscope and equipped for imaging standard slides, culture dishes, and microplates (e.g. 96-well plates).

Overview

Laboratories for Molecular Medicine, Room 111

TI2The Nikon Ti2-E fluorescence microscope is a high-content imaging system, based on an inverted microscope and equipped for imaging standard slides, culture dishes, and microplates (e.g. 96-well plates).  The microscope includes 'Perfect Focus' for autofocus applications, a Photometrics Prime 95B sCMOS camera for imaging fluorescence, a 16 megapixel Nikon DS-Ri2 color camera for imaging histology slides, a motorized stage and NIS-Elements High Content (HC) software for multiwell plate acquisition.

Operation

Objectives:  

  • 4x    Plan Apochromat Lambda, N.A. 0.2, W.D. 20 mm, F.O.V. 25 mm
  • 10x  Plan Fluor Phase Contrast DL, N.A. 0.3, W.D. 16 mm, F.O.V. 25 mm
  • 20x  Super Plan Fluor ELWD, N.A. 0.45, W.D. 6.9-8.2 mm, F.O.V. 22 mm, DIC, Correction Collar 0-2 mm
  • 40x  Super Plan Fluor ELWD, N.A. 0.6, W.D. 2.8-3.6 mm, F.O.V. 22 mm, DIC, Correction Collar 0-2 mm
  • 60x  Oil Plan Apochromat Lambda Phase Contrast DM, N.A. 1.4, W.D. 0.13 mm, F.O.V. 25 mm, Ph3

Resources

Reservations

Microscopes in the Bioimaging Facility can be reserved using iLab calendars. All facility users are invited to use the system, which requires a one-time registration.

Contact