Division of Biology and Medicine
BioMed Core Facilities

FACSAriaIIIu Flow Cytometer and Cell Sorter

FACSAriaIIIThe BD FACSAriaIIIu is a sorter that incorporates a fixed-alignment cuvette flow cell. This new flow cell provides superior fluorescence sensitivity. The FACSAriaIIIu has fice air-cooled lasers at 355nm, 407nm, 488nm, 561nm, and 633nm allowing for multicolor analysis of up to 20  parameters. The FACSAria can sort 1, 2, 3, or 4 populations simultaneously and is equipped with an Automated Cell Deposition Unit for sorting into multiwell plates or slides. The FACSAriaIIIu is housed in a Baker Bioprotect E3 hood with an Aerosol Management System that evacuates any aerosols from the sort chamber, providing an additional level of safety for the operator while maintaining sterility for samples. The FACSAriaIIIu also has a refrigeration system that allows cooling of the sort sample as well as the sort collection tubes. It can accept various sample input tubes including 1 mL microtubes, 12 x 75-mm test tubes, and 15 mL tubes into the sample and collection chambers.

Operation

Resources

Publications

The following acknowledgement must appear in any publication made possible by the work of the Flow Cytometry and Sorting Facility.

This project was supported in part by a grant from the National Center For Research Resources (NCRR) (1S 10RR021051) from the National Institutes of Health, Brown University's Division of Biology and Medicine and Provost's office.

Reservations

Dodard G, Tata A, Erick TK, Jaime D, Miah SMS, Quatrini L, Escalière B, Ugolini S, Vivier E, Brossay L. Inflammation-Induced Lactate Leads to Rapid Loss of Hepatic Tissue-Resident NK Cells. Cell Rep. 2020 Jul 7;32(1):107855. doi: 10.1016/j.celrep.2020.107855. PMID: 32640221; PMCID: PMC7383148.

Gura MA, Mikedis MM, Seymour KA, de Rooij DG, Page DC, Freiman RN. Dynamic and regulated TAF gene expression during mouse embryonic germ cell development. PLoS Genet. 2020 Jan 8;16(1):e1008515. doi: 10.1371/journal.pgen.1008515. PMID: 31914128; PMCID: PMC7010400.

Crane MJ, Daley JM, van Houtte O, Brancato SK, Henry WL Jr, Albina JE. The monocyte to macrophage transition in the murine sterile wound. PLoS One. 2014 Jan 22;9(1):e86660. doi: 10.1371/journal.pone.0086660. PMID: 24466192; PMCID: PMC3899284.

Duwaerts CC, Sun EP, Cheng CW, van Rooijen N, Gregory SH. Cross-activating invariant NKT cells and kupffer cells suppress cholestatic liver injury in a mouse model of biliary obstruction. PLoS One. 2013 Nov 15;8(11):e79702. doi: 10.1371/journal.pone.0079702. PMID: 24260285; PMCID: PMC3829879.

Jain S, Webster TJ, Sharma A, Basu B. Intracellular reactive oxidative stress, cell proliferation and apoptosis of Schwann cells on carbon nanofibrous substrates. Biomaterials. 2013 Jul;34(21):4891-901. doi: 10.1016/j.biomaterials.2013.03.055. Epub 2013 Apr 6. PMID: 23570716.

Jeyapalan JC, Sedivy JM. How to measure RNA expression in rare senescent cells expressing any specific protein such as p16Ink4a. Aging (Albany NY). 2013 Feb;5(2):120-9. doi: 10.18632/aging.100536. PMID: 23454889; PMCID: PMC3616199.

Duwaerts CC, Gehring S, Cheng CW, van Rooijen N, Gregory SH. Contrasting responses of Kupffer cells and inflammatory mononuclear phagocytes to biliary obstruction in a mouse model of cholestatic liver injury. Liver Int. 2013 Feb;33(2):255-65. doi: 10.1111/liv.12048. Epub 2012 Dec 13. PMID: 23240869; PMCID: PMC3540118.

Gil MP, Ploquin MJ, Watford WT, Lee SH, Kim K, Wang X, Kanno Y, O'Shea JJ, Biron CA. Regulating type 1 IFN effects in CD8 T cells during viral infections: changing STAT4 and STAT1 expression for function. Blood. 2012 Nov 1;120(18):3718-28. doi: 10.1182/blood-2012-05-428672. Epub 2012 Sep 11. PMID: 22968462; PMCID: PMC3488885.

Reilly EC, Thompson EA, Aspeslagh S, Wands JR, Elewaut D, Brossay L. Activated iNKT cells promote memory CD8+ T cell differentiation during viral infection. PLoS One. 2012;7(5):e37991. doi: 10.1371/journal.pone.0037991. Epub 2012 May 23. PMID: 22649570; PMCID: PMC3359346.

Zhang L, Webster TJ. Poly-lactic-glycolic-acid surface nanotopographies selectively decrease breast adenocarcinoma cell functions. Nanotechnology. 2012 Apr 20;23(15):155101. doi: 10.1088/0957-4484/23/15/155101. Epub 2012 Mar 22. PMID: 22436863.

Zhang L, Webster TJ. Decreased lung carcinoma cell functions on select polymer nanometer surface features. J Biomed Mater Res A. 2012 Jan;100(1):94-102. doi: 10.1002/jbm.a.33217. Epub 2011 Oct 11. PMID: 21987490.

Tessmer MS, Reilly EC, Brossay L. Salivary gland NK cells are phenotypically and functionally unique. PLoS Pathog. 2011 Jan 13;7(1):e1001254. doi: 10.1371/journal.ppat.1001254. PMID: 21249177; PMCID: PMC3020929.

Venet F, Huang X, Chung CS, Chen Y, Ayala A. Plasmacytoid dendritic cells control lung inflammation and monocyte recruitment in indirect acute lung injury in mice. Am J Pathol. 2010 Feb;176(2):764-73. doi: 10.2353/ajpath.2010.090765. Epub 2009 Dec 30. PMID: 20042672; PMCID: PMC2808083.

Venet F, Chung CS, Huang X, Lomas-Neira J, Chen Y, Ayala A. Lymphocytes in the development of lung inflammation: a role for regulatory CD4+ T cells in indirect pulmonary lung injury. J Immunol. 2009 Sep 1;183(5):3472-80. doi: 10.4049/jimmunol.0804119. Epub 2009 Jul 29. PMID: 19641139; PMCID: PMC2788796.

Holub M, Cheng CW, Mott S, Wintermeyer P, van Rooijen N, Gregory SH. Neutrophils sequestered in the liver suppress the proinflammatory response of Kupffer cells to systemic bacterial infection. J Immunol. 2009 Sep 1;183(5):3309-16. doi: 10.4049/jimmunol.0803041. Epub 2009 Jul 29. PMID: 19641138.

Kelly JR, Rubin AJ, Davis JH, Ajo-Franklin CM, Cumbers J, Czar MJ, de Mora K, Glieberman AL, Monie DD, Endy D. Measuring the activity of BioBrick promoters using an in vivo reference standard. J Biol Eng. 2009 Mar 20;3:4. doi: 10.1186/1754-1611-3-4. PMID: 19298678; PMCID: PMC2683166.

Wintermeyer P, Cheng CW, Gehring S, Hoffman BL, Holub M, Brossay L, Gregory SH. Invariant natural killer T cells suppress the neutrophil inflammatory response in a mouse model of cholestatic liver damage. Gastroenterology. 2009 Mar;136(3):1048-59. doi: 10.1053/j.gastro.2008.10.027. Epub 2008 Nov 1. PMID: 19056387; PMCID: PMC2654188.

Wesley JD, Tessmer MS, Chaukos D, Brossay L. NK cell-like behavior of Valpha14i NK T cells during MCMV infection. PLoS Pathog. 2008 Jul 18;4(7):e1000106. doi: 10.1371/journal.ppat.1000106. PMID: 18636102; PMCID: PMC2442879.

For further information or to schedule an appointment time, contact Kevin Carlson at 401-863-6396 or send us an email.

Email Kevin Carlson     Email the Flow Cytometry Facility

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