Division of Biology and Medicine
BioMed Core Facilities
Drosophila Media Facility
The Drosophila Media Facility is dedicated to providing investigators with high quality media for Drosophila research.
Drosophila Media Facility
The Drosophila Media Facility is dedicated to providing investigators with high quality media for Drosophila research.
About
The Drosophila Media Facility is dedicated to providing investigators with high quality media for Drosophila research. In the centralized kitchen, a standard cornmeal, sucrose, yeast medium is prepared and dispensed into different sized culture vessels. Media is prepared according to a strict protocol that ensures consistency in diet and the absence of any bacterial, fungal or insect contamination. Researchers using glass vials return them to the facility where they are autoclaved, washed, and filled again.
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Services and Instruments
| Product | Quantity |
|---|---|
| Tray of 10ml Glass Vials | 140 vials/tray |
| Tray of 10ml Narrow Plastic Vials | 100 vials/tray |
| Tray of 12ml Wide Plastic Vials | 100 vials/tray |
| 40ml Plastic Bottle | Each |
Drosophila Media Recipes
We prepare 4 different types of media according to the recipes listed below. Modified recipes may be requested based on the formulas listed below.
| K Winter Media | |
|---|---|
| Ingredient | Recipe amount for 1 liter of media |
| Water (l) | 1.00 |
| Agar (g) | 7.66 |
| Yeast (g) | 29.44 |
| Cornmeal (g) | 55.63 |
| Sugar (g) | 117.27 |
| Tego (g) | 2.55 |
| Ethanol (ml) | 9.78 |
| K Summer Media | |
| Ingredient | Recipe amount for 1 liter of media |
| Water (l) | 1.00 |
| Agar (g) | 8.43 |
| Yeast (g) | 32.39 |
| Cornmeal (g) | 61.19 |
| Sugar (g) | 129.00 |
| Tego (g) | 2.81 |
| Ethanol (ml) | 9.78 |
| T Media | |
| Ingredient | Recipe amount for 1 Rack |
| Water (l) | 0.85 |
| Agar (g) | 7.9 |
| SAF Yeast (g) | 24.75 |
| Cornmeal (g) | 52 |
| Sugar (g) | 110 |
| Tego (g) | 2.38 |
| 95% Ethanol (ml) | 9.2 |
| W Winter Media | |
| Ingredient | Recipe amount for 1 liter of media |
| Water (l) | 1.0 |
| Agar (g) | 7.2 |
| Yeast (g) | 29.4 |
| Cornmeal (g) | 55.6 |
| Sugar (g) | 117.3 |
| Tego (g) | 2.6 |
| 95% Ethanol (ml) | 9.8 |
Equipment
The Drosophila Media Facility is equipped with a food service quality steam kettle fitted with stirring unit, a Steris autoclave and an electric heat glassware washer. Media is dispensed using Filamatic and Materflex/Digistalic pumps. Media is prepared in a steam-jacketed food kettle and pumped into trays of glass vials (10ml), plastic vials (10ml) or into plastic bottles (40ml) depending on research needs.
1
of
5
Kettle
2
of
5
RoboFiller
3
of
5
Glass Vials
4
of
5
Autoclave
5
of
5
Washer
Service Request and Reservations
For information on using this facility, please contact Chuck Toth.
Rates
FY25 Rates
| Media Prep Rates | ||
|---|---|---|
| Plug Costs | ||
| Service | Units | Internal Academic Rates* |
| Glass Vials (100 vials/tray) | Tray | $4 |
| Glass Vials | Each | $0.04 |
| Tray of 10ml Plastic Vials, Narrow Polypropylene (100 vials/tray) | Tray | $22 |
| 10ml Plastic Vials, Narrow Polypropylene | Each | $0.22 |
| Tray of 10ml Plastic Vials, Narrow Ploystyrene (100 vials/tray) | Tray | $23 |
| 10ml Plastic Vials, Narrow Polystyrene | Each | $0.23 |
| Tray of 12ml Plastic Vials, Wide Polystyrene (100 vials/tray) | Tray | $28 |
| 12ml Plastic Vials, Wide | Each | $0.28 |
| 40ml Plastic Bottle with Buzz Plug | Bottle | $0.78 |
| Technical Assistance | Hours | $30 |
| Supply | Unit | Rate |
| Droso-Plugs For Tray of Narrow Vials (100) | Tray | $6.82 |
| Droso-Plugs For Tray of Wide Vials | Tray | $9.28 |
| Bag of Droso-Plugs, Wide Vials (1210) | Bag | $112.25 |
| Bag of Droso-Plugs, Narrow Vials (1,690) | Bag | $115.30 |
| Buzz Plugs - 40ml Plastic Bottle | Each | $0.20 |
| Supply costs are variable and subject to change without notice. | ||
*Rates for Brown and Rhode Island Academic and Hospital Affiliates.
Rates Effective 11/18/2024
Contact/Location
The Drosophila Media Prep Facility is located in the BioMed Center.
Resources for Grants
Drosophila Media Prep Facility. This facility is located at the Biomedical Center and is overseen by the Director of the Multidisciplinary Teaching Laboratories and staffed by a full-time technical assistant. The Facility provides investigators with high quality media for Drosophila research and genetic stock maintenance. To provide large and small quantities of media, this centralized kitchen is equipped with a food-service quality Cleveland Range Kettle MKET-12-T. Media is pumped into trays of vials or plastic bottles using a Gilson Fly Food Dispenser. Used glass vials are returned to the facility where they are sterilized in a Steris autoclave and washed in a Northwestern Systems G403 electric heat glassware washer.
Scientific reproducibility is enhanced through scientific rigor and transparency. Scientific rigor is the strict application of the scientific method to ensure unbiased and well-controlled experimental design, methodology, analysis, interpretation, and reporting of results. The Drosophila Media Prep Facility is committed to supporting research excellence by adopting the following practices of scientific rigor.
- Purchase and maintain a variety of high-quality instruments from established vendors such that the best instrument is available for any given research analysis.
- The equipment is overseen by highly trained experts and well maintained under service contracts or funds budgeted for annual preventive maintenance and repairs.
- The media prep experts are available for experimental design consults or troubleshooting.
- Standard operating procedures are followed for media preparation.
Acknowledgment
This facility was supported in part by Brown University's Division of Biology and Medicine and Provost's office.