Division of Biology and Medicine
BioMed Core Facilities
Zeiss LSM 800 Confocal Laser Scanning Microscope
Optical sectioning of samples labeled with fluorescent indicators
Zeiss LSM 800 Confocal Laser Scanning Microscope
Optical sectioning of samples labeled with fluorescent indicators
Overview
Sidney Frank Hall, Room 115
The Zeiss LSM800 is an upright Confocal Laser Scanning Microscope, which is well-suited for imaging microscope slides. This microscope can image fluorescence in thin optical slices and stacks of images can be acquired to examine fluorescent structures in 3D. The system is based on a Axio Imager Z2 microscope and is equipped with a URGB laser module (Diode lasers: 405, 488, 561 and 640 nm), high-quality objectives (10x, 20x, 40x water, 40x oil, 63x oil) and three sensitive GaAsP detectors.
Operation
Specifications
Objectives on the LSM 800:
|
Mag |
Correction |
NA |
DIC |
WD (mm) |
HFW @ 1x |
|---|---|---|---|---|---|
|
10x |
PlanNEOFL |
0.3 |
none |
5.2 |
(non-confocal obj) |
|
20x |
Plan Apochromat |
0.8 |
DIC II |
0.55 |
319.5 µm |
|
40x Water |
C Apochromat |
1.2 |
DIC III |
0.28 |
159.7 µm |
|
40x Oil |
Plan Apochromat |
1.3 |
DIC III |
0.21 |
159.7 µm |
|
63x Oil |
Plan Apochromat |
1.4 |
DIC III |
0.19 |
101.4 µm |
Resources
Zeiss
Reservations
Reserve a microscope using the iLab calendars
Reserve a microscope using the iLab calendars
https://brown.ilab.agilent.com/service_center/show_external/5278/bioimaging_facility