The Zeiss Axiovert 200M Fluorescence Microscope is a motorized inverted microscope with an AxioCam MRc5 color camera and a Hamamatsu ORCA-ER monochrome camera controlled by AxioVision 4.8.2 software. This microscope is well equipped for transmitted light microscopy and fluorescence microscopy.

Objectives:

Theoretical XY Objective Resolution: based on: Dxy=0.61*wavelength/NA

Transmitted light microscopy: The Axiovert 200M has DIC and Phase Contrast on all objectives (Plan Neo 5x, 10x, 20x, 40x dry, 40x oil, and 100x oil). The high resolution color camera can be used for imaging fixed samples (e.g. histological preparations) and for time-lapse recordings of living cells. A stage heater is available for imaging living cells at a set temperature.

Fluorescence microscopy: The filter sets allow imaging of a wide variety of fluorescent indicators such as Alexa-Fluors, Calcium-Green, Cy2, Cy3, Cy5, DAPI, Fluorescein (FITC), FM1-43, Fluo-3, Fluo-4, GFP, CFP, YFP, Hoechst 33258, Rhodamine, Texas Red, TRITC, and others. The stage heater and the sensitive monochrome camera make this microscope particularly well suited for imaging dynamic patterns of fluorescence in living cells.

Filter sets on the Axiovert 200M:
1) DIC: Analyzer for DIC
2) DAPI: Chroma set 49028, Excitation 384-407 nm, Emission 435-485 nm (bandpass filter).
3) FITC: Chroma set 41001, Excitation 460-500 nm, Emission 510-560 nm (bandpass filter).
4) RHOD: Chroma set 41002c, Excitation 530-560 nm, Emission 590-650 nm (bandpass filter).
5) Cy5: Chroma set 41024, Excitation 590-650 nm, Emission 665 nm and above (long pass filter).

Also available upon request:
6) GFP: Chroma set 41017, Excitation 450-490 nm, Emission 500-550 nm (bandpass filter).
7) CFP: Chroma set 31044v2, Excitation 426-446 nm, Emission 460-500 nm (bandpass filter).
8) YFP: Chroma set 41028, Excitation 490-510 nm, Emission 520-550 nm (bandpass filter).
9) CFP/YFP FRET: Chroma set 31052, Excitation 426-446 nm, Emission 520-550 nm.